Binding properties of a phosphonate clip towards nucleic acid building blocks
Binding properties of a phosphonate clip towards nucleic acid building blocks
Raditzky, B.; Eilzer, M.
Abstract
Water-soluble clips are possible molecules to study the weak, non-covalent interactions, responsible for many biological processes. The complexation behaviour of naphthalene clips, substituted with phosphonate or phosphate groups, has been studied by time-resolved laser-induced fluorescence spectroscopy with ultrafast pulses (fs-TRLFS). As guest molecules we used nucleic bases, nucleosides and nucleotides. The determined association constants for the 1:1 complexes range from log Ka = 3.9 4.2 and are around one order of magnitude higher than published in literature. The measured spectra assumes the appearance of excited state species or dimerization of the clip.
Keywords: moleculare clips; nuclic bases; nucleosides; nucleotides; fs-TRLFS; association
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Vortrag (Konferenzbeitrag)
4th SUPRAPHONE/3rd WG Meeting COST D31, 31.05.-03.06.2007, Lipari, Italy
Permalink: https://www.hzdr.de/publications/Publ-10190