Publikationsrepositorium - Helmholtz-Zentrum Dresden-Rossendorf

Bacterial isolates from the uranium mining waste pile Haberland (Johanngeorgenstadt, Saxony) possess high affinities to heavy metals and uranium. This binding effect is caused by the components of the bacterial cell wall, mainly effected by surface layer proteins.
Aim of this work is the construction of a stable multi-layer system for the simulation of cell walls of Gram-positive bacteria and the investigation of metal interactions with single compounds and whole complex systems.
The quartz crystal microbalance (QCM-D) and atomic force microscopy (AFM) is used to track and control this multi-layer formation.
First result of these tasks is the proof of recrystallization of S-layer proteins as part of the cell wall of Bacillus spec. JG-B53 via QCM-D and AFM.