Automation of the radiosynthesis and purification procedures for [¹⁸F]Fluspidine preparation, a new radiotracer for clinical investigations in PET imaging of σ₁ receptors in brain.

The radiosynthesis of [¹⁸F]Fluspidine, a potent σ₁ receptor imaging probe for preclinical/clinical studies, was implemented on a TRACERlabTM FX F-N synthesizer. [¹⁸F]2 was synthesized in 15 min at 85 °C starting from its tosylate precursor. Purification via semipreparative RP-HPLC was investigated using different columns and eluent compositions and was most successful on a polar RP phase with acetonitrile/water buffered with NH4OAc.
After solid phase extraction, [¹⁸F]Fluspidine was formulated and produced within 59 ± 4 min with an overall radiochemical yield of 37 ± 8%, a radiochemical purity of 99.3 ± 0.5% and high specific activity (176.6 ± 52.0 GBq/μmol).1 receptor imaging probe for preclinical/clinical studies, was implemented on a TRACERlabTM FX F-N synthesizer. [¹⁸F]2 was synthesized in 15 min at 85 °C starting from its tosylate precursor. Purification via semipreparative RP-HPLC was investigated using different columns and eluent compositions and was most successful on a polar RP phase with acetonitrile/water buffered with NH4OAc.
After solid phase extraction, [¹⁸F]Fluspidine was formulated and produced within 59 ± 4 min with an overall radiochemical yield of 37 ± 8%, a radiochemical purity of 99.3 ± 0.5% and high specific activity (176.6 ± 52.0 GBq/μmol).