Publikationsrepositorium - Helmholtz-Zentrum Dresden-Rossendorf
1 PublikationNon-Thermal Induction of Conforamtional Rearrangment in Proteins by Far-Infrared Excitation
Lörincz, I. Z.; Schay, G.; Rauscher, A. A.; Kellermayer, M. S. Z.; Gensch, M.; Malnasi-Csizmadia, A.
Abstract
How does a protein respond to infrared excitation? Our aim was to induce a specific non-thermal conformational change of a protein by infrared laser excitation. The fluorescence intensity of an intrinsically fluorescent protein, LSS-mOrange was monitored upon irradiation with a free electron laser at two of its IR absorption peaks (11.36/9.56 microns) and at a wavelength (9.06 microns) where the protein has no absorption. The irradiation at the absorption peaks caused a reversible fluorescence intensity increase on the sec-min timescale.
Beteiligte Forschungsanlagen
- Hochfeld-Magnetlabor (HLD)
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Biophysical Journal 106(2014)2, 460a
DOI: 10.1016/j.bpj.2013.11.2608
Permalink: https://www.hzdr.de/publications/Publ-21666