Simplified Expression and Production of Small Metal Binding Peptides


Simplified Expression and Production of Small Metal Binding Peptides

Braun, R.; Matys, S.; Schoenberger, N.; Lederer, F.; Pollmann, K.

Abstract

Phage display for discovery of metal binding peptides is an innovative way to engineer metalsorptive biological structures with a broad spectrum of applications in geobiotechnology, for example in reusable filter modules in biosorption processes. Using state-of-the-art cloning techniques we developed an easy-to-use cloning and expression system, allowing the fast production of identified peptides.

Keywords: phage display; intein; protein engineering; Gibson Assembly; biomining; bioremediation; biosorption

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Permalink: https://www.hzdr.de/publications/Publ-25553