Publikationsrepositorium - Helmholtz-Zentrum Dresden-Rossendorf

A marine, facultatively anaerobic, nitrogen-fixing bacterium, designated strain DNF-1T, was isolated from the lagoon
sediment of Dongsha Island, Taiwan. Cells grown in broth cultures were Gram-negative rods that were motile by means of
monotrichous flagella. Cells grown on plate medium produced prosthecae and vesicle-like structures. NaCl was required and
optimal growth occurred at about 2–3% NaCl, 25–30 °C and pH 7–8. The strain grew aerobically and was capable of
anaerobic growth by fermenting D-glucose or other carbohydrates as substrate. Both the aerobic and anaerobic growth
could be achieved with NH4Cl as a sole nitrogen source. When N2 served as the sole nitrogen source only anaerobic
growth was observed. Major cellular fatty acids were C14:0, C16:0 and C16:1ω7c, while major polar lipids were
phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 42.2 mol% based on the genomic DNA
data. Phylogenetic analyses based on 16S rRNA genes and the housekeeping genes, gapA, pyrH, recA and gyrB, revealed
that the strain formed a distinct lineage at species level in the genus Vibrio of the family Vibrionaceae. These results and
those from genomic, chemotaxonomic and physiological studies strongly support the assignment of a novel Vibrio species.
The name Vibrio salinus sp. nov. is proposed for the novel species, with DNF-1T (= BCRC 81209T = JCM 33626T) as the
type strain. This newly proposed species represents the second example of the genus Vibrio that has been demonstrated to
be capable of anaerobic growth by fixing N2 as the sole nitrogen source